4ZNJ

Thermus Phage P74-26 Large Terminase ATPase domain mutant R139A (I 2 3 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-17
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	I 2 3
Unit cell lengths	129.578, 129.578, 129.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.975 - 2.532
R-factor	0.2012
Rwork	0.197
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zni
RMSD bond length	0.006
RMSD bond angle	0.887
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.980	2.622
High resolution limit [Å]	2.532	2.532
Rmerge		2.214
Number of reflections	11395
<I/σ(I)>	45.99	2.59
Completeness [%]	100.0	100
Redundancy	43.1	42.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.5M ammonium sulfate and 0.1 mM tri-sodium citrate pH 4.5