4ZNI
Thermus Phage P74-26 Large Terminase ATPase domain (I 2 3 space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 129.578, 129.578, 129.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.813 - 2.097 |
| R-factor | 0.1761 |
| Rwork | 0.173 |
| R-free | 0.21320 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.813 | 2.171 |
| High resolution limit [Å] | 2.097 | 2.097 |
| Rmerge | 0.087 | 1.275 |
| Number of reflections | 21390 | |
| <I/σ(I)> | 15.8 | 2.48 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.7 M ammonium sulfate and 0.1 M tri-sodium citrate pH 5.0 |
