4ZNG
X-ray crystallography of recombinant Lactococcus lactis prolidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-11 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 212.130, 76.990, 88.920 |
| Unit cell angles | 90.00, 112.39, 90.00 |
Refinement procedure
| Resolution | 49.830 - 2.250 |
| R-factor | 0.2227 |
| Rwork | 0.220 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wy2 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.830 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.084 | 0.800 |
| Number of reflections | 63058 | |
| <I/σ(I)> | 9.48 | 1.74 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | calcium acetate, sodium cacodylate, PEG 8000, glycerol |
