4ZNE
IgG1 Fc-FcgammaRI ecd complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.735, 126.750, 71.844 |
| Unit cell angles | 90.00, 118.44, 90.00 |
Refinement procedure
| Resolution | 86.550 - 2.420 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.566 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.550 | 2.430 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.476 | |
| Number of reflections | 39992 | |
| <I/σ(I)> | 14.4 | 2.4 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 295 | 50 mM Zinc acetate dehydrate and 20% PEG 3350 |
