4ZN2
Glycosyl hydrolase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.559, 88.206, 222.394 |
Unit cell angles | 90.00, 96.92, 90.00 |
Refinement procedure
Resolution | 47.120 - 2.000 |
R-factor | 0.1954 |
Rwork | 0.193 |
R-free | 0.22410 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.031 |
Data reduction software | SCALEPACK |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 143014 | |
<I/σ(I)> | 21.85 | 3.87 |
Completeness [%] | 99.4 | 99 |
Redundancy | 5.4 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 3%(w/v) Dextran sulfate sodium salt, 0.1 M BICINE, 7%(w/v) PEG20000. |