4ZN2
Glycosyl hydrolase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.559, 88.206, 222.394 |
| Unit cell angles | 90.00, 96.92, 90.00 |
Refinement procedure
| Resolution | 47.120 - 2.000 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.22410 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.031 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 143014 | |
| <I/σ(I)> | 21.85 | 3.87 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 3%(w/v) Dextran sulfate sodium salt, 0.1 M BICINE, 7%(w/v) PEG20000. |
