4ZN1

Crystal Structure of MjSpt4:Spt5 complex conformation A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-20
Detector	MAR CCD 165 mm
Wavelength(s)	1.000
Spacegroup name	P 4
Unit cell lengths	84.820, 84.820, 34.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.930 - 2.800
R-factor	0.24392
Rwork	0.242
R-free	0.28247
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ewg 1ryq 1npp
RMSD bond length	0.009
RMSD bond angle	1.426
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.930	2.950
High resolution limit [Å]	2.800	2.800
Rmerge		0.299
Number of reflections	6086
<I/σ(I)>	20.6	3.7
Completeness [%]	99.6	99.9
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	PEG 5000MME, propanol, MES, sarcosine