4ZMZ
Crystal structure of human P-cadherin (monomer 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.660, 40.740, 72.860 |
| Unit cell angles | 90.00, 94.66, 90.00 |
Refinement procedure
| Resolution | 32.420 - 2.050 |
| R-factor | 0.21819 |
| Rwork | 0.216 |
| R-free | 0.26629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zmn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.294 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.081 | 0.288 |
| Number of reflections | 13000 | |
| <I/σ(I)> | 9.4 | 3.3 |
| Completeness [%] | 92.2 | 83.4 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 28% PEG 400 200 mM CaCl2 95 mM HEPES |
