4ZMY
Crystal structure of human P-cadherin (monomer 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.840, 40.910, 72.480 |
| Unit cell angles | 90.00, 97.27, 90.00 |
Refinement procedure
| Resolution | 26.220 - 1.500 |
| R-factor | 0.13953 |
| Rwork | 0.137 |
| R-free | 0.18606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zmn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.509 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.220 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.429 |
| Number of reflections | 32358 | |
| <I/σ(I)> | 13.1 | 2.2 |
| Completeness [%] | 91.2 | 64.8 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 4,000 100 mM CaCl2 100 mM TRIS |
