4ZMW
Crystal structure of human P-cadherin (enc-X-dimer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.369, 106.810, 77.579 |
| Unit cell angles | 90.00, 94.86, 90.00 |
Refinement procedure
| Resolution | 47.020 - 2.300 |
| R-factor | 0.23277 |
| Rwork | 0.230 |
| R-free | 0.27558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zmn |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.592 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.020 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.066 | 0.279 |
| Number of reflections | 33629 | |
| <I/σ(I)> | 15.4 | 4.8 |
| Completeness [%] | 98.8 | 92 |
| Redundancy | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.15 M Lithium sulfate 7.5 mM nickel sulfate 100 mM TRIS |
