4ZMU
Dcsbis, a diguanylate cyclase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.609, 109.083, 111.850 |
| Unit cell angles | 90.00, 95.88, 90.00 |
Refinement procedure
| Resolution | 42.259 - 2.502 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.24560 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.246 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 58687 | |
| <I/σ(I)> | 19.86 | 1.81 |
| Completeness [%] | 96.6 | 77.8 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 10% PEG8000, 0.1 M HEPES pH 7.5, 8% ethylene glycol |
