4ZMS
Structure of the full-length response regulator spr1814 in complex with a phosphate analogue and B3C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9198 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.980, 115.526, 50.073 |
| Unit cell angles | 90.00, 92.41, 90.00 |
Refinement procedure
| Resolution | 37.817 - 1.900 |
| R-factor | 0.1673 |
| Rwork | 0.165 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.772 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 35816 |
| <I/σ(I)> | 4.7 |
| Completeness [%] | 96.7 |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1M sodium citrate tribasic dihydrate pH 5.0, 30%(v/v) Jeffamine ED-2001 pH 7 |
