4ZMQ
Crystal structure of human P-cadherin (ss-X-dimer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 182.090, 182.090, 41.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.720 - 2.200 |
| R-factor | 0.18861 |
| Rwork | 0.186 |
| R-free | 0.23112 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zmn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.603 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.720 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 | 0.387 |
| Number of reflections | 37841 | |
| <I/σ(I)> | 18.2 | 4.4 |
| Completeness [%] | 94.6 | 70.7 |
| Redundancy | 9.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 28% PEG 400 250 mM CaCl2 HEPES 100 mM |
