4ZMO
Crystal structure of human P-cadherin (ss-dimer K14E)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.380, 81.230, 48.990 |
| Unit cell angles | 90.00, 105.79, 90.00 |
Refinement procedure
| Resolution | 42.430 - 2.480 |
| R-factor | 0.20046 |
| Rwork | 0.199 |
| R-free | 0.23549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zmn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.598 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.430 | 2.610 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.061 | 0.426 |
| Number of reflections | 15628 | |
| <I/σ(I)> | 13.8 | 2.5 |
| Completeness [%] | 98.3 | 91.3 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 26% PEG 4,000 170 lithium sulfate 15% glycerol 85 mM TRIS |
