4ZML
Crystal structure of human P-cadherin (ss-dimer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.950, 80.910, 49.180 |
| Unit cell angles | 90.00, 105.75, 90.00 |
Refinement procedure
| Resolution | 40.460 - 1.850 |
| R-factor | 0.19621 |
| Rwork | 0.195 |
| R-free | 0.21775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zmn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.764 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.460 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.066 | 0.635 |
| Number of reflections | 38255 | |
| <I/σ(I)> | 14.5 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 31% PEG 400 15 mM CaCl2 5% glycerol |
