4ZMJ
Crystal Structure of Ligand-Free BG505 SOSIP.664 HIV-1 Env Trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 107.180, 107.180, 103.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.083 - 3.310 |
| R-factor | 0.2676 |
| Rwork | 0.266 |
| R-free | 0.28550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tvp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.310 | 3.300 |
| Rmerge | 0.089 | 0.500 |
| Number of reflections | 7041 | |
| <I/σ(I)> | 18.5 | 1.16 |
| Completeness [%] | 68.3 | 11.2 |
| Redundancy | 5.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 26% PEG 400, 3.2% PEG 3350, and 0.1M sodium acetate pH 5.5 |
