4ZMI

Crystal structure of the Helical domain of S. pombe Taz1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000, 0.97898, 0.97953, 0.98341
Spacegroup name	C 2 2 2
Unit cell lengths	68.010, 160.102, 50.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.107 - 2.300
R-factor	0.1956
Rwork	0.194
R-free	0.23290
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.166
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.300
High resolution limit [Å]	2.250	5.550	2.250
Rmerge	0.043	0.033	0.373
Total number of observations	87731
Number of reflections	13012
<I/σ(I)>	19.7
Completeness [%]	96.6	84.2	80.3
Redundancy	6.7	6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	277	100 mM Tris-HCl pH7.0, 18% PEG 3350, 300mM KNO3, 10 mM Co(NH4)6Cl3, 10 mM DTT