4ZMI
Crystal structure of the Helical domain of S. pombe Taz1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000, 0.97898, 0.97953, 0.98341 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 68.010, 160.102, 50.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.107 - 2.300 |
R-factor | 0.1956 |
Rwork | 0.194 |
R-free | 0.23290 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.166 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.300 |
High resolution limit [Å] | 2.250 | 5.550 | 2.250 |
Rmerge | 0.043 | 0.033 | 0.373 |
Total number of observations | 87731 | ||
Number of reflections | 13012 | ||
<I/σ(I)> | 19.7 | ||
Completeness [%] | 96.6 | 84.2 | 80.3 |
Redundancy | 6.7 | 6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 277 | 100 mM Tris-HCl pH7.0, 18% PEG 3350, 300mM KNO3, 10 mM Co(NH4)6Cl3, 10 mM DTT |