4ZMH

Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	116.442, 124.294, 180.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.148 - 1.930
R-factor	0.1399
Rwork	0.138
R-free	0.17010
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.171
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (dev_1834)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.930
Number of reflections	188525
<I/σ(I)>	19
Completeness [%]	97.7
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M sodium dihydrogen phosphate, 20%PEG3350, 0.1M citric acid