4ZM7
PcCel45A N105D mutatnt at cryo condition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.764, 57.955, 63.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.970 - 0.701 |
| R-factor | 0.1268 |
| Rwork | 0.126 |
| R-free | 0.13470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.710 |
| High resolution limit [Å] | 0.700 | 0.700 |
| Rmerge | 0.092 | 0.301 |
| Number of reflections | 262663 | |
| <I/σ(I)> | 90.4 | 6.4 |
| Completeness [%] | 95.1 | 85.1 |
| Redundancy | 14.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20 mM Tris-HCl, 65% 3-methyl-1,5-pentanediol |
