4ZM6
A unique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain GH3 beta-N-acetylglucosaminidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 245.034, 245.034, 94.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.630 - 2.800 |
| R-factor | 0.23039 |
| Rwork | 0.229 |
| R-free | 0.25404 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.042 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.190 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.012 | |
| Number of reflections | 71082 | |
| <I/σ(I)> | 29.4 | 4.3 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.3-1.6 M (NH4)2SO4 , 0.1 M Bis-Tris pH 5.5-6.5 |
