4ZM2
Antitoxin Phd from phage P1 in complex with its operator DNA inverted repeat in a monoclinic space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.870, 56.580, 74.840 |
Unit cell angles | 90.00, 111.47, 90.00 |
Refinement procedure
Resolution | 43.920 - 3.880 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.27090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.450 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.900 | 4.020 |
High resolution limit [Å] | 3.880 | 3.880 |
Number of reflections | 4674 | |
<I/σ(I)> | 4.63 | 1.55 |
Completeness [%] | 99.5 | |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 35% 1,4-Dioxane and 200 mM NaCl, 100 mM Tris pH 7.5 |