4ZM2
Antitoxin Phd from phage P1 in complex with its operator DNA inverted repeat in a monoclinic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.870, 56.580, 74.840 |
| Unit cell angles | 90.00, 111.47, 90.00 |
Refinement procedure
| Resolution | 43.920 - 3.880 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.450 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 4.020 |
| High resolution limit [Å] | 3.880 | 3.880 |
| Number of reflections | 4674 | |
| <I/σ(I)> | 4.63 | 1.55 |
| Completeness [%] | 99.5 | |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 35% 1,4-Dioxane and 200 mM NaCl, 100 mM Tris pH 7.5 |
