4ZLN
Crystal structure of transporter AcrB deletion mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 143.853, 143.853, 519.714 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.953 - 3.557 |
| R-factor | 0.2711 |
| Rwork | 0.269 |
| R-free | 0.30330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9b |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.747 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.100 | 3.760 |
| High resolution limit [Å] | 3.557 | 3.557 |
| Number of reflections | 25237 | |
| <I/σ(I)> | 4.2 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M ADA buffer at pH 7.4, 0.1 M Li2SO4, 10% PEG 3350 |
