4ZLK
Crystal structure of mouse myosin-5a in complex with calcium-bound calmodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.079, 99.455, 248.159 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.849 - 2.502 |
R-factor | 0.1989 |
Rwork | 0.196 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oe9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.157 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.106 |
Number of reflections | 47736 |
<I/σ(I)> | 2 |
Completeness [%] | 97.4 |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Tris-HCl, pH7.5, 2 mM CaCl2, 2 mM DTT, 2 mM NaN3, 10% glycerol, 6% PEG 8000 |