4ZLJ
Crystal structure of transporter AcrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 144.989, 144.989, 521.112 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.973 - 3.257 |
| R-factor | 0.2516 |
| Rwork | 0.248 |
| R-free | 0.31780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.582 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.200 | 3.370 |
| High resolution limit [Å] | 3.257 | 3.257 |
| Number of reflections | 33386 | |
| <I/σ(I)> | 6.3 | 2 |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 15.3 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M ADA buffer at pH 7.4, 0.1 M Li2SO4, 10% PEG 3350 |
