4ZLC

Crystal structure of the ROQ domain of human Roquin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	60.760, 60.868, 169.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.438 - 2.700
R-factor	0.22596
Rwork	0.224
R-free	0.26009
RMSD bond length	0.010
RMSD bond angle	1.430
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	17819
<I/σ(I)>	14.4
Completeness [%]	99.1
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	15% PEG6000, 100 mM Hepes, 100 mM KCl