4ZL8
Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.280, 63.680, 41.980 |
| Unit cell angles | 90.00, 98.26, 90.00 |
Refinement procedure
| Resolution | 34.914 - 1.395 |
| R-factor | 0.1467 |
| Rwork | 0.146 |
| R-free | 0.15940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h93 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.294 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.5.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.545 | 1.470 |
| High resolution limit [Å] | 1.395 | 1.395 |
| Number of reflections | 36539 | |
| <I/σ(I)> | 9.7 | 3.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20-29 % PEG 1500, 0.1 M MES |
