4ZKY

Structure of F420 binding protein, MSMEG_6526, from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	56.704, 59.415, 95.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.396 - 1.650
R-factor	0.2242
Rwork	0.222
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qvb
RMSD bond length	0.007
RMSD bond angle	1.114
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.810	1.670
High resolution limit [Å]	1.650	1.650
Rmerge	0.057	1.011
Number of reflections	39312
<I/σ(I)>	21.3	1.4
Completeness [%]	98.3	76.8
Redundancy	7	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Sodium Iodide, 22% Peg 3350