4ZKU
P22 Tail Needle Gp26 crystallized at pH 10.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2008-05-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 3 |
| Unit cell lengths | 43.200, 43.200, 271.234 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.820 - 2.500 |
| R-factor | 0.191 |
| Rwork | 0.184 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2poh |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.655 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_2016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.099 | 0.242 |
| Number of reflections | 19166 | |
| <I/σ(I)> | 13.66 | 4.4 |
| Completeness [%] | 96.9 | 84.6 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 40% PEG 4000, 0.1M CAPS pH 10.0 |
