4ZKQ
Viral chemokine binding protein R17 encoded by rodent gammaherpesvirus Peru ( RHVP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.561, 75.835, 106.985 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.011 - 1.898 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.22080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | The 2.7 Angstrom model resolved by SAD experiment |
RMSD bond length | 0.005 |
RMSD bond angle | 0.996 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | REFMAC |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.650 | |
Number of reflections | 45516 | |
<I/σ(I)> | 20.8 | 1.83 |
Completeness [%] | 93.8 | 61.2 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris pH 8.5 20% PEG550 MME |