4ZKI

The crystal structure of Histidine Kinase YycG with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	53.499, 96.034, 119.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.819 - 3.401
R-factor	0.2025
Rwork	0.201
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u7o
RMSD bond length	0.011
RMSD bond angle	1.437
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.400
Number of reflections	8921
<I/σ(I)>	10
Completeness [%]	99.8
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	298	1.0 M Ammonium Sulfate, 1% PEG 3350