4ZKB
The chemokine binding protein of orf virus complexed with CCL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 93.2 |
Detector technology | CCD |
Collection date | 2011-03-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95369 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.000, 78.000, 186.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.440 - 2.900 |
R-factor | 0.27041 |
Rwork | 0.268 |
R-free | 0.32302 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p5i |
RMSD bond length | 0.009 |
RMSD bond angle | 1.444 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.440 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.117 | 0.757 |
Number of reflections | 13416 | |
<I/σ(I)> | 8.4 | 1.8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.9 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 0.2M potassium sodium tartrate tetrahydrate, 0.1M sodium citrate tribasic dihydrate pH5.6, 2.0M ammonium citrate |