4ZKB
The chemokine binding protein of orf virus complexed with CCL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 93.2 |
| Detector technology | CCD |
| Collection date | 2011-03-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.000, 78.000, 186.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.440 - 2.900 |
| R-factor | 0.27041 |
| Rwork | 0.268 |
| R-free | 0.32302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p5i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.444 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.440 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.117 | 0.757 |
| Number of reflections | 13416 | |
| <I/σ(I)> | 8.4 | 1.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 0.2M potassium sodium tartrate tetrahydrate, 0.1M sodium citrate tribasic dihydrate pH5.6, 2.0M ammonium citrate |
