4ZJG
Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning domains MG0-NIE-MG1.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792, 0.9795, 0.9688 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.870, 70.870, 146.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.800 - 2.300 |
| R-factor | 0.2147 |
| Rwork | 0.213 |
| R-free | 0.25630 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.800 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 1.083 |
| Number of reflections | 17299 | |
| <I/σ(I)> | 22.9 | 3.4 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 25.4 | 24.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 30% [v/v] PEG400 100mM Tris-HCl |
