4ZJF
Crystal structure of GP1 - the receptor binding domain of Lassa virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.620, 86.470, 109.480 |
| Unit cell angles | 90.00, 92.81, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.595 |
| R-factor | 0.2121 |
| Rwork | 0.209 |
| R-free | 0.24130 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.862 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1683) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 2.680 |
| High resolution limit [Å] | 2.595 | 2.590 |
| Rmerge | 0.102 | 1.139 |
| Number of reflections | 28667 | |
| <I/σ(I)> | 14.3 | 1.6 |
| Completeness [%] | 99.1 | 94.2 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M BisTris pH 5.0, 0.2 M ammonium acetate pH 7.0, 22 % (w/v) PEG 6000 and 5 % (v/v) PEG 200 |
