4ZJD
Small heat shock protein AgsA from Salmonella typhimurium: Truncations at N- and C- termini
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 89.780, 89.780, 707.483 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.140 - 7.500 |
| R-factor | 0.354 |
| Rwork | 0.351 |
| R-free | 0.39600 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.919 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.610 | 7.910 |
| High resolution limit [Å] | 7.500 | 7.500 |
| Rmerge | 0.948 | |
| Number of reflections | 1625 | |
| <I/σ(I)> | 8 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30 % Polypropylene glycol 400, 0.1 M NaCl |
