4ZJA
Small heat shock protein AgsA from Salmonella typhimurium: C-terminal truncated construct
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | I 2 3 |
Unit cell lengths | 126.400, 126.400, 126.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.689 - 4.101 |
R-factor | 0.387 |
Rwork | 0.386 |
R-free | 0.41270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zj9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.539 |
Data reduction software | MOSFLM (7.0.9) |
Data scaling software | SCALA |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.200 | 4.320 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmerge | 0.010 | |
Number of reflections | 2757 | |
<I/σ(I)> | 14.8 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.6 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% Pentaerythritol propoxylate (5/4 PO/OH), 0.1M MES-NaOH, 30% glycerol |