4ZJ7

Crystal structure of the karyopherin Kap121p bound to the extreme C-terminus of the protein phosphatase Cdc14p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-14
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	77.263, 127.758, 131.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.814 - 2.400
R-factor	0.2206
Rwork	0.219
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w3x
RMSD bond length	0.003
RMSD bond angle	0.774
Data reduction software	MOSFLM
Data scaling software	Aimless (0.3.11)
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.360	26.360	2.470
High resolution limit [Å]	2.400	9.900	2.400
Rmerge	0.106	0.038	0.599
Rpim	0.058	0.023	0.333
Total number of observations	220860	2223	18360
Number of reflections	51446
<I/σ(I)>	7.2	18.8	1.6
Completeness [%]	99.5	80.9	99.7
Redundancy	4.3	3.4	4.2
CC(1/2)	0.991	0.971	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	PEG 20000, 2-propanol