4ZJ0
The crystal structure of monomer Q108K:K40L:Y60W CRBPII bound to all-trans-retinal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 194 |
| Detector technology | CCD |
| Collection date | 2010-10-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.081, 36.049, 63.920 |
| Unit cell angles | 90.94, 91.99, 113.93 |
Refinement procedure
| Resolution | 21.300 - 1.500 |
| R-factor | 0.20076 |
| Rwork | 0.198 |
| R-free | 0.25071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcq |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.317 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.300 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 41751 | |
| <I/σ(I)> | 50.3 | 3.58 |
| Completeness [%] | 92.8 | 68 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 30% PEG4000, 0.1M CH3COONa.3H2O, pH 4.6, 0.1M CH3COONH4 |
