4ZIX

Structure of HEWL using Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	273
Detector technology	PIXEL
Collection date	2014-02-27
Detector	CS-PAD CXI-1
Wavelength(s)	1.56
Spacegroup name	P 43 21 2
Unit cell lengths	79.100, 79.100, 38.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.966 - 1.890
R-factor	0.1665
Rwork	0.165
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.713
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	27.970
High resolution limit [Å]	1.890
Number of reflections	10266
<I/σ(I)>	9.5
Completeness [%]	100.0
Redundancy	26558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3.5	293	Lysozyme powder from chicken egg white was dissolved in 0.02 M Sodium acetate pH 4.6 buffer to a final concentration of 50 mg/mL. Crystallization was initiated by injecting 20 uL of lysozyme solution (50 mg/mL) into 1 mL of precipitant solution (20 %(w/v) NaCl, 6 %(v/v) PEG 6000, 1 M Na acetate pH 3.0) at room temperature.