4ZIG

Crystal Structure of core/latch dimer of Bax in complex with BidBH3mini

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	100.120, 100.120, 37.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.959 - 2.200
R-factor	0.1832
Rwork	0.181
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bd2
RMSD bond length	0.008
RMSD bond angle	0.981
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	2.278
High resolution limit [Å]	2.200	2.200
Rmerge		0.845
Number of reflections	10108
<I/σ(I)>	15.18	2.61
Completeness [%]	100.0	100
Redundancy	7.8	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	Tri-sodium citrate, Sodium cacodylate