4ZIF
Crystal Structure of core/latch dimer of Bax in complex with BimBH3mini
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 96.140, 96.140, 37.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.766 - 2.401 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bd2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.770 | 2.486 |
| High resolution limit [Å] | 2.401 | 2.401 |
| Rmerge | 0.058 | 0.792 |
| Number of reflections | 7219 | |
| <I/σ(I)> | 22.48 | 2.61 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | Tri-sodium citrate, Sodium cacodylate |
