4ZI9
Structure of mouse clustered PcdhgA1 EC1-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.751, 63.687, 107.486 |
Unit cell angles | 90.00, 99.69, 90.00 |
Refinement procedure
Resolution | 33.510 - 1.700 |
R-factor | 0.2307 |
Rwork | 0.230 |
R-free | 0.26630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wcp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.510 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.128 | |
Number of reflections | 81585 | |
<I/σ(I)> | 5.42 | 0.33 |
Completeness [%] | 80.0 | 36 |
Redundancy | 5.42 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris pH 8.5, 7.5% PEG8000, 200 mM NDSB-221 |