4ZHY

Crystal structure of a bacterial signalling complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	118.539, 160.989, 43.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.887 - 1.969
R-factor	0.1833
Rwork	0.181
R-free	0.21860
RMSD bond length	0.008
RMSD bond angle	1.054
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.969
Number of reflections	29376
<I/σ(I)>	29.2
Completeness [%]	97.6
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, 1.8M ammonium sulfate