4ZHU

Crystal structure of a bacterial repressor protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	121.268, 121.268, 85.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.543 - 2.397
R-factor	0.1829
Rwork	0.181
R-free	0.21130
RMSD bond length	0.009
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.397
Number of reflections	25665
<I/σ(I)>	20.2
Completeness [%]	99.1
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, 1.5M lithium sulfate monohydrate