4ZHO
The crystal structure of Arabidopsis ferredoxin 2 with 2Fe-2S cluster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.74 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 60.730, 60.730, 154.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.730 - 2.340 |
| R-factor | 0.19831 |
| Rwork | 0.197 |
| R-free | 0.21582 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.933 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.730 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.066 | 0.598 |
| Number of reflections | 12894 | |
| <I/σ(I)> | 23.2 | 4.2 |
| Completeness [%] | 99.4 | 98.4 |
| Redundancy | 12.3 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.2 M MgCl2, 0.1 M Tris, 20 % PEG 8000 |
