4ZHJ
Crystal Structure of the Catalytic Subunit of Magnesium Chelatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | H 3 |
Unit cell lengths | 319.717, 319.717, 105.194 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.959 - 2.502 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.20920 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.873 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.085 | 0.820 |
Number of reflections | 138183 | |
<I/σ(I)> | 16.7 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.85 M sodium citrate, 2 mM dithiothreitol |