4ZHB
N-terminal structure of ankyrin repeat-containing protein legA11 from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 42.708, 42.708, 145.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.710 - 1.300 |
| R-factor | 0.1277 |
| Rwork | 0.126 |
| R-free | 0.15880 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.395 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
| Rmerge | 0.074 | 0.047 | 0.882 |
| Total number of observations | 405549 | ||
| Number of reflections | 31841 | ||
| <I/σ(I)> | 12.9 | ||
| Completeness [%] | 92.5 | 97.5 | 57.5 |
| Redundancy | 12.7 | 12.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1 M MES pH 6.5, 12% PEG 20000 |
