4ZH5
Crystal structure of Endoglucanase from Perinereis brevicirris with Cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.150, 108.170, 112.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.980 - 1.350 |
R-factor | 0.11665 |
Rwork | 0.115 |
R-free | 0.14812 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wc3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.610 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.3.6) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.980 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.065 | 0.532 |
Number of reflections | 203725 | |
<I/σ(I)> | 9.1 | 2 |
Completeness [%] | 98.0 | 97.1 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 100 mM Bris-Tris, pH5.5, 300 mM NaCl, 25% PEG 3350 |