4ZGU
Crystal structure of monomer Y60W hCRBPII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2013-10-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.596, 54.335, 68.588 |
| Unit cell angles | 108.13, 97.03, 104.09 |
Refinement procedure
| Resolution | 30.010 - 1.490 |
| R-factor | 0.20076 |
| Rwork | 0.198 |
| R-free | 0.24558 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.323 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.030 | 0.408 |
| Number of reflections | 77520 | |
| <I/σ(I)> | 47.91 | |
| Completeness [%] | 98.9 | 89.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294.15 | 30% PEG 4000, 0.1M CH3COONa.3H2O pH4.6, 0.1M CH3COONH4 |
