4ZGS
Identification of the pyruvate reductase of Chlamydomonas reinhardtii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.760, 110.920, 193.160 |
Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
Resolution | 66.840 - 2.461 |
R-factor | 0.205 |
Rwork | 0.202 |
R-free | 0.25410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dld |
RMSD bond length | 0.011 |
RMSD bond angle | 1.521 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.840 | 2.520 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.071 | 0.367 |
Number of reflections | 126464 | |
<I/σ(I)> | 8.6 | 1.8 |
Completeness [%] | 98.7 | 99.2 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 1:1 10mg/ml protein with 0.2M sodium malonate pH 6.0 20% w/v PEG 3350 |