4ZGR
Structural studies on a non-toxic homologue of type II RIPs from Momordica charantia (bitter gourd) in complex with T-Antigen.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.370, 135.800, 44.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.260 - 1.970 |
| R-factor | 0.17958 |
| Rwork | 0.179 |
| R-free | 0.19769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z8s |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.516 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 2.070 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.085 | |
| Number of reflections | 63830 | |
| <I/σ(I)> | 14.5 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1M HEPES, 20% w/v PEG 10000, glycerol |
