4ZGQ
Structure of Cdc123 bound to eIF2-gammaDIII domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.000, 117.400, 132.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.000 |
| R-factor | 0.2078 |
| Rwork | 0.206 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.170 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 1.150 | |
| Number of reflections | 12020 | |
| <I/σ(I)> | 13.4 | 2 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 7.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 25%PEG3350, 0.2M LISO4, 0.1M Tris pH 8.0 |
